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(2023)Cite this article
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Fine-tuning gene expression is crucial for generating quantitative phenotypic changes and crop improvement. Using CRISPR–Cas base editing and prime editing, we engineered upstream open reading frames — eukaryotic translational control elements — in rice to incrementally downregulate translation to predictable and desired levels.
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References
Gao, C. Genome engineering for crop improvement and future agriculture. Cell 184, 1621–1635 (2021). A review of the development and application of genome editing tools in plants.
Bowman, E. K. et al. Bidirectional titration of yeast gene expression using a pooled CRISPR guide RNA approach. Proc. Natl Acad. Sci. USA 117, 18424–18430 (2020). A plasmid-based library methodology for bidirectional, graded modulation of gene expression in yeast.
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Zhang, H. et al. Genome editing of upstream open reading frames enables translational control in plants. Nat. Biotechnol. 36, 894–898 (2018). A method for upregulating protein expression by editing uORFs.
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This is a summary of: Xue, C. et al. Tuning plant phenotypes by precise, graded downregulation of gene expression. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01707-w (2023).
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Engineered upstream open reading frames predictably downregulate mRNA translation in plants.
Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01729-4
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DOI: https://doi.org/10.1038/s41587-023-01729-4
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Margherita Wrona