Science & Nature
- Research Briefing
- Published:
(2023)Cite this article
Subjects
Nano-tRNAseq is a nanopore-based, cost-effective and high-throughput approach to quantify transfer RNA (tRNA) abundances and modifications simultaneously, providing a framework to study the ‘tRNAome’ at single-molecule resolution. We envision that Nano-tRNAseq will enable us to study the role of tRNA molecules and their modifications in a wide variety of contexts.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 per month
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Rent or buy this article
Get just this article for as long as you need it
$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Torres, A. G., Batlle, E. & Ribas de Pouplana, L. Role of tRNA modifications in human diseases. Trends Mol. Med. 20, 306–314 (2014). A comprehensive review of the human tRNA modifications and their links to disease.
Goodarzi, H. et al. Modulated expression of specific tRNAs drives gene expression and cancer progression. Cell 165, 1416–1427 (2016). This work showed that specific tRNAs are upregulated in breast cancer cells with increased metastatic potential.
Zhang, W., Foo, M., Eren, A. M. & Pan, T. tRNA modification dynamics from individual organisms to metaepitranscriptomics of microbiomes. Mol. Cell 82, 891–906 (2022). A review of tRNA modification dynamics, NGS-based methods for tRNA quantification, and the potential of using tRNA modifications to study biological communities such as microbiomes.
Thomas, N. K. et al. Direct nanopore sequencing of individual full length tRNA strands. ACS Nano. 15, 16642–16653 (2021). This work used nanopore DRS to sequence native tRNA molecules.
Begik, O. et al. Quantitative profiling of pseudouridylation dynamics in native RNAs with nanopore sequencing. Nat. Biotechnol. 39, 1278–1291 (2021). This work shows that RNA modifications can be detected as base-calling errors in nanopore DRS, but that their signatures differ depending on the modification type and sequence context.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Lucas, M. C. et al. Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01743-6 (2023).
Rights and permissions
About this article
Cite this article
Exploring tRNAs and their modifications and crosstalk using Nano-tRNAseq.
Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01755-2
Published:
DOI: https://doi.org/10.1038/s41587-023-01755-2
Read More
Lyndia Menjivar