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(2023)Cite this article
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Leveraging advances in hardware and probes, we have combined innovations in optics and algorithms to allow automated single-molecule-based super-resolution imaging at unprecedented throughput. This approach allows us to obtain nanoscale information from large cell populations, bridging the gap between imaging and indirect ensemble methods.
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References
Lieberman-Aiden, E. et al. Comprehensive mapping of long-range interactions reveals folding principles of the human genome. Science 326, 289–293 (2009). This paper describes a powerful biochemical method for inferring cellular nanostructure.
Liu, S., Hoess, P. & Ries, J. Super-resolution microscopy for structural cell biology. Ann. Rev. Biophys. 51, 301–326 (2022). A review article on super-resolution microscopy.
Lelek, M. G. et al. Single-molecule localization microscopy. Nat. Rev. Methods Primers 1, 39 (2021). A review article on single-molecule-based super-resolution.
Pepperkok, R. & Ellenberg, J. High-throughput fluorescence microscopy for systems biology. Nat. Rev. Mol. Cell Biol. 7, 690–696 (2006). An early review of high-throughput microscopy methods.
Manley, S., Gillette, J. M. & Lippincott-Schwartz, J. Single-particle tracking photoactivated localization microscopy for mapping single-molecule dynamics. Methods Enzymol. 475, 109–120 (2010). The original paper reporting dense single-particle tracking.
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This is a summary of: Barentine, A. E. S. et al. An integrated platform for high-throughput nanoscopy. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01702-1 (2023).
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Imaging large cell populations with fast, automated super-resolution.
Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01711-0
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DOI: https://doi.org/10.1038/s41587-023-01711-0
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Elida Byron