{"id":608713,"date":"2023-02-16T20:49:22","date_gmt":"2023-02-17T02:49:22","guid":{"rendered":"https:\/\/news.sellorbuyhomefast.com\/index.php\/2023\/02\/16\/single-cell-mapping-of-combinatorial-target-antigens-for-car-switches-using-logic-gates\/"},"modified":"2023-02-16T20:49:22","modified_gmt":"2023-02-17T02:49:22","slug":"single-cell-mapping-of-combinatorial-target-antigens-for-car-switches-using-logic-gates","status":"publish","type":"post","link":"https:\/\/newsycanuse.com\/index.php\/2023\/02\/16\/single-cell-mapping-of-combinatorial-target-antigens-for-car-switches-using-logic-gates\/","title":{"rendered":"Single-cell mapping of combinatorial target antigens for CAR switches using logic gates"},"content":{"rendered":"

Science & Nature <\/p>\n

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Data availability<\/h2>\n

The list of cell surface proteins was obtained from the in silico human surfaceome database at http:\/\/wlab.ethz.ch\/surfaceome\/<\/a> (ref. 23<\/a><\/sup>). The tumor-normal single-cell meta-atlas is available at https:\/\/cellatlas.kaist.ac.kr\/cart<\/a>. Raw and processed datasets used in this study are deposited at the Zenodo repository (https:\/\/doi.org\/10.5281\/zenodo.7416669<\/a>)50<\/a><\/sup>. Ovarian cancer single-cell transcriptome data generated in this work are available on the Gene Expression Omnibus with accession number GSE192898<\/a> (ref. 51<\/a><\/sup>). Source data<\/a> are provided with this paper.<\/p>\n<\/div>\n

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Code availability<\/h2>\n

Source codes for CAR target antigen identification, named PCASA (prioritization of combinatorial cancer-associated surface antigens), are available on GitHub at https:\/\/github.com\/kaistomics\/PCASA<\/a> (ref. 52<\/a><\/sup>).<\/p>\n<\/div>\n

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    Download references<\/a><\/p>\n<\/div>\n<\/div>\n

    \n

    Acknowledgements<\/h2>\n

    This work was supported by the Bio & Medical Technology Development Program of the National Research Foundation, funded by the Ministry of Science & ICT (NRF-2017M3A9A7050612, NRF-2019M3A9B6064688, NRF-2019M3A9B6064691 and NRF-2021M3A9I402444711), and also supported by the Korea Health Technology R&D Project of the Korea Health Industry Development Institute, funded by the Ministry of Health & Welfare (HI16C1559).<\/p>\n<\/div>\n

    \n

    Author information<\/h2>\n
    \n

    Author notes<\/span><\/p>\n

      \n
    1. \n

      These authors contributed equally: Joonha Kwon, Junho Kang.<\/p>\n<\/li>\n<\/ol>\n

      Authors and Affiliations<\/h3>\n
        \n
      1. \n

        Department of Bio and Brain Engineering, KAIST, Daejeon, Republic of Korea<\/p>\n

        Joonha Kwon,\u00a0Kayoung Seo,\u00a0Dohyeon An,\u00a0Jun Hyeong Lee\u00a0&\u00a0Jung Kyoon Choi<\/p>\n<\/li>\n

      2. \n

        Graduate School of Medical Science and Engineering, KAIST, Daejeon, Republic of Korea<\/p>\n

        Junho Kang,\u00a0Mert Yakup Baykan\u00a0&\u00a0Jong-Eun Park<\/p>\n<\/li>\n

      3. \n

        Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea<\/p>\n

        Areum Jo,\u00a0Nayoung Kim,\u00a0Hye Hyeon Eum\u00a0&\u00a0Hae-Ock Lee<\/p>\n<\/li>\n

      4. \n

        Department of Biomedicine and Health Sciences, Graduate School, The Catholic University of Korea, Seoul, Republic of Korea<\/p>\n

        Areum Jo,\u00a0Nayoung Kim,\u00a0Hye Hyeon Eum\u00a0&\u00a0Hae-Ock Lee<\/p>\n<\/li>\n

      5. \n

        Department of Pathology, CHA Bundang Medical Center, CHA University, Seongnam-si, Republic of Korea<\/p>\n

        Sohyun Hwang\u00a0&\u00a0Hee Jung An<\/p>\n<\/li>\n

      6. \n

        Department of Biomedical Science, CHA University, Pocheon-si, Republic of Korea<\/p>\n

        Sohyun Hwang<\/p>\n<\/li>\n

      7. \n

        CHA Advanced Research Institute, CHA Bundang Medical Center, Seongnam-si, Republic of Korea<\/p>\n

        Ji Min Lee<\/p>\n<\/li>\n

      8. \n

        Samsung Genome Institute, Samsung Medical Center, Seoul, Republic of Korea<\/p>\n

        Woong-Yang Park<\/p>\n<\/li>\n

      9. \n

        Penta Medix Co., Ltd., Seongnam-si, Republic of Korea<\/p>\n

        Jung Kyoon Choi<\/p>\n<\/li>\n<\/ol>\n

        Contributions<\/h3>\n

        Conceptualization: J. Kwon, J. Kang, J.E.P. and J.K.C. Methodology: J. Kwon, J. Kang, A.J., K.S., D.A., M.Y.B., J.H.L., N.K., H.H.E., J.M.L. and W.Y.P. Investigation: J. Kwon, J. Kang, A.J., K.S., D.A., N.K. and H.H.E. Visualization: J. Kwon, J. Kang and M.Y.B. Funding acquisition: J.K.C. Project administration: H.J.A., J.E.P. and J.K.C. Supervision: S.H., H.J.A., H.O.L., J.E.P. and J.K.C. Writing\u2014original draft: J. Kwon, J. Kang and J.H.L. Writing\u2014review and editing: J. Kwon, J. Kang, J.E.P. and J.K.C.<\/p>\n

        Corresponding authors<\/h3>\n

        Correspondence to
        \n Hee Jung An<\/a>, Hae-Ock Lee<\/a>, Jong-Eun Park<\/a> or Jung Kyoon Choi<\/a>.<\/p>\n<\/div>\n<\/div>\n

        \n

        Ethics declarations<\/h2>\n
        \n

        Competing interests<\/h3>\n

        The authors declare no competing interests.<\/p>\n<\/p><\/div>\n<\/div>\n

        \n

        Peer review<\/h2>\n
        \n

        Peer review information<\/h3>\n

        Nature Biotechnology<\/i> thanks Raphael Gottardo, Peter Linsley and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.<\/p>\n<\/p><\/div>\n<\/div>\n

        \n

        Additional information<\/h2>\n

        Publisher\u2019s note<\/b> Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.<\/p>\n<\/div>\n

        \n

        Extended data<\/h2>\n
        \n
        \n

        Extended Data Fig. 1 UMAP representation of the normal cell atlas overlaid with tumor cells and tumor-infiltrating normal cells from selected tumor datasets.<\/a><\/h3>\n

        . a<\/b>, Same plot as Fig. 1e<\/a> but with cell-type annotation instead of tissue origin information. b<\/b>, Same plot as Fig. 1e<\/a> but showing representative cells selected during tumor cellpreserving subsampling by geometric sketching.<\/p>\n<\/div>\n

        \n

        Extended Data Fig. 2 Tumor versus normal differences in ECFs and expression levels.<\/a><\/h3>\n

        Differences of ECFs (a<\/b>) and expression levels (b<\/b>) between tumor and normal cellsfor surfaceome genes ordered by the FI values from the RF model in selected cancer types.<\/p>\n<\/div>\n

        \n

        Extended Data Fig. 3 Comparison of cancer types with expression level and FI for surfaceome genes.<\/a><\/h3>\n

        a<\/b>, Clustering heatmap displaying the expression level of surface antigens according to the cancer type. Five major clusters (C1 ~ C5) of genes were denoted. b<\/b>, Comparison of the different cancer types based on the correlation of the FI values from the cancer type-specific RF model.<\/p>\n<\/div>\n

        \n

        Extended Data Fig. 4 Tumor-specific ECF values of subtype marker genes in BRCA samples for which clinical subtype information was available.<\/a><\/h3>\n
        \n

        The boxplot shows the ECF status of three markers, containing ERBB2, ESR1, and PGR, for all subtypes (ERpositive: n\u2009=\u200911, HER2-positive: n\u2009=\u20098, HER2 and ER double positive: n\u2009=\u20094, TNBC: n\u2009=\u200912): TNBC, Triple-negative breast cancer; \u2018+\u2018 means \u2018positive\u2019. Average values of ECFs for ERBB2, ESR1, and PGR, were 0.33\u2009\u00b1\u20090.15 (s.d.), 0.68\u2009\u00b1\u20090.15 (s.d), and 0.29\u2009\u00b1\u20090.18 (s.d.) in ER-positive subtype while HER2-positive subtype showed 0.86\u2009\u00b1\u20090.19 (s.d.), 0.18\u2009\u00b1\u20090.3 (s.d.), and 0.01\u2009\u00b1\u20090.01 (s.d.), respectively. In the case of HER2+ ER+, average values were 0.47\u2009\u00b1\u20090.19 (s.d.), 0.25\u2009\u00b1\u20090.23 (s.d.), and 0.06\u2009\u00b1\u20090.05 (s.d.), and the TNBC subtype showed 0.24\u2009\u00b1\u20090.14 (s.d), 0.05\u2009\u00b1\u20090.12 (s.d.), and 0.05\u2009\u00b1\u20090.15 (s.d.), for ERBB2, ESR1, and PGR, respectively. Data in the box plot represent the first quartile (25%), median (center), and third quartile (75%) with minimum and maximum values. Black points indicate the outliers.<\/p>\n

        \n Source data<\/a>\n <\/p>\n<\/div>\n<\/div>\n

        \n

        Extended Data Fig. 5 Correlation between the CNN weight and ECF of each antigen combination in PAAD, LIHC, NSCLC, CRC, and BRCA.<\/a><\/h3>\n
        \n

        For each of the pairs, the GradCAM weight returned by the CNN model was mapped to the ECF among tumor cells (blue) and normal cells (red). The Pearson correlation coefficient and two-sided P values are shown at the top. Translucent bands around the regression lines represent the confidence interval (CI) of 95%.<\/p>\n

        \n Source data<\/a>\n <\/p>\n<\/div>\n<\/div>\n

        \n

        Extended Data Fig. 6 Comparison of tumor versus normal ECFs according to the CNN weight in PAAD, LIHC, NSCLC, CRC, and BRCA.<\/a><\/h3>\n

        Tumor and normal ECFs of the combinations were ordered by their CNN weight. The combinations meeting the ECF criteria (> 70% tumor and < 10% normal) were highlighted in color.<\/p>\n<\/div>\n

        \n

        Extended Data Fig. 7 Sample-wise co-expression of selected combinations in OV.<\/a><\/h3>\n
        \n

        Expression correlations of the OV gene pairs were computed by using pan-cancer TCGA samples (n\u2009=\u200911,768) and GTEx normal samples (n\u2009=\u200917,382). The Pearson correlation coefficient was used.<\/p>\n

        \n Source data<\/a>\n <\/p>\n<\/div>\n<\/div>\n

        \n

        Extended Data Fig. 8 Across-sample expression patterns of selected logical CAR switches in PAAD, LIHC, NSCLC, CRC, and BRCA.<\/a><\/h3>\n
        \n

        Shown are the top 10 gene combinations with the largest CNN weight for each cancer type and each logic switch (AND, OR, and NOT). The heatmap intensity scales with the combinatorial tumor (left) and normal (right) ECF values.<\/p>\n

        \n Source data<\/a>\n <\/p>\n<\/div>\n<\/div>\n

        \n

        Extended Data Fig. 9 Additional single-cell epitope analysis results for EPCAM and CD24 in ovarian cancer.<\/a><\/h3>\n

        a<\/b>, Distribution of EPCAM and CD24 protein expression levels broken down by annotated cell type. Log2 fold changes comparing CITE-seq ADT signals from targeting antibodies versus isotype controls were computed and are displayed in each cell type. b<\/b>, Clustering and cell-type mapping of tumor and tumor-resident normal cells based on the CITE-seq transcriptome. c<\/b>, Single-cell maps, as in (b), overlaid with mRNA expression levels retrieved from CITEseq. d<\/b>, Single-cell maps, as in (b), overlaid with protein expression levels retrieved from CITE-seq. e<\/b>, Single-cell maps, as in (b), overlaid with the mRNA expression status indicating cells with both EPCAM and CD24 (brown), only EPCAM (light blue), only CD24 (light red), or none (gray). f<\/b>, Single-cell maps, as in (b), overlaid with the epitope expression status indicating cells with both EPCAM and CD24 (brown), only EPCAM (light blue), only CD24 (light red), or none (gray).<\/p>\n<\/div>\n

        \n

        Extended Data Fig. 10 Single-cell epitope analysis of validation targets in colorectal cancer.<\/a><\/h3>\n

        a<\/b>, Clustering and cell-type mapping of the tumor and tumor-infiltrating normal cells based on the CITE-seq transcriptome of our pooled CRC samples. b<\/b>, Single-cell maps, as in (a), overlaid with mRNA expression levels retrieved from the CITE-seq experiments. c<\/b>, Single-cell maps, as in (b), overlaid with protein expression levels retrieved from the CITE-seq experiments. d<\/b>, Distribution of protein expression levels broken down by annotated cell type. Log2 fold changes comparing CITE-seq ADT signals from targeting antibodies versus isotype controls were computed and are displayed for each protein in each cell type. e<\/b>, Cell-by-cell protein expression patterns in the tumor and tumor-resident normal cells for CEACAM5 versus EPCAM, CEACAM5 versus CA4, CEACAM5 versus CPM, and CEACAM5 versus VSIG2. Cells enclosed in the marked zones indicate the expression of both proteins for the AND gating and only one protein (CEACAM5) for the NOT gating.<\/p>\n<\/div>\n<\/div>\n<\/div>\n

        \n

        Supplementary information<\/h2>\n
        \n

        \n

        Reporting Summary<\/a><\/h3>\n<\/p>\n
        \n

        Supplementary Tables 1\u20139<\/a><\/h3>\n

        Supplementary Table 1: Summary of our cancer cell atlas. Supplementary Table 2: Status of cell types for tumor datasets integrated with normal cell atlas. Supplementary Table 3: Summary of selected tumor datasets of six cancer types. Supplementary Table 4: Performance of RF cell classifier for each cancer type. Supplementary Table 5: RF FI for top 100 genes for each cancer type. Supplementary Table 6: CNN weights calculated by Grad-CAM for each cancer type. Supplementary Table 7: Performance of CNN cell classifier for each cancer type. Supplementary Table 8: Sample-wise co-expression for OV target combinations. Supplementary Table 9: Normal ECF distribution by cell types for OV target combinations.<\/p>\n<\/div>\n<\/div>\n<\/div>\n

        \n

        Source data<\/h2>\n<\/div>\n