{"id":602603,"date":"2023-01-29T14:50:25","date_gmt":"2023-01-29T20:50:25","guid":{"rendered":"https:\/\/news.sellorbuyhomefast.com\/index.php\/2023\/01\/29\/clash-enables-large-scale-parallel-knock-in-for-cell-engineering\/"},"modified":"2023-01-29T14:50:25","modified_gmt":"2023-01-29T20:50:25","slug":"clash-enables-large-scale-parallel-knock-in-for-cell-engineering","status":"publish","type":"post","link":"https:\/\/newsycanuse.com\/index.php\/2023\/01\/29\/clash-enables-large-scale-parallel-knock-in-for-cell-engineering\/","title":{"rendered":"CLASH enables large-scale parallel knock-in for cell engineering"},"content":{"rendered":"\n<div>\n<header>\n<ul data-test=\"article-identifier\">\n<li data-test=\"article-category\">Research Briefing<\/li>\n<li><a href=\"http:\/\/www.nature.com\/#article-info\" data-track=\"click\" data-track-action=\"publication date\" data-track-label=\"link\">Published: <time datetime=\"2023-01-26\">26 January 2023<\/time><\/a><\/li>\n<\/ul>\n<ul data-test=\"authors-list\" data-component-authors-activator=\"authors-list\"><\/ul>\n<p data-container-section=\"info\">\n<p>    <a data-test=\"journal-link\" href=\"http:\/\/www.nature.com\/nbt\"><i data-test=\"journal-title\">Nature Biotechnology<\/i><\/a><\/p>\n<p>                         (<span data-test=\"article-publication-year\">2023<\/span>)<a href=\"http:\/\/www.nature.com\/#citeas\" data-track=\"click\" data-track-action=\"cite this article\" data-track-label=\"link\">Cite this article<\/a>\n                    <\/p>\n<div>\n<ul>\n<li>\n<p>369 <span>Accesses<\/span><\/p>\n<\/li>\n<li>\n<p>1 <span>Altmetric<\/span><\/p>\n<\/li>\n<li>\n<p><a href=\"http:\/\/www.nature.com\/articles\/s41587-023-01664-4\/metrics\" data-track=\"click\" data-track-action=\"view metrics\" data-track-label=\"link\" rel=\"nofollow\">Metrics <span>details<\/span><\/a><\/p>\n<\/li>\n<\/ul><\/div>\n<\/header>\n<div data-component=\"article-subject-links\">\n<h3>Subjects<\/h3>\n<\/p><\/div>\n<\/p><\/div>\n<div>\n<p>CLASH is a CRISPR-based platform that enables the parallel knock-in via homology-directed repair of a large pool of transgene variants encoded in adeno-associated virus vectors. CLASH can be applied to the systematic and unbiased selection of favorable features in T cells and, in principle, other cell types.<\/p>\n<div>\n<p>This is a preview of subscription content, <a href=\"https:\/\/wayf.springernature.com?redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41587-023-01664-4\" data-track=\"click\" data-track-action=\"institution access preview subscription\" data-track-label=\"link\">access via your institution<\/a><\/p>\n<\/p><\/div>\n<h2 id=\"access-options\">Access options<\/h2>\n<section>\n<div>\n<p>Subscribe to Nature+<\/p>\n<p>Get immediate online access to Nature and 55 other Nature journal<\/p>\n<\/div>\n<div>\n<p>Subscribe to Journal<\/p>\n<p>Get full journal access for 1 year<\/p>\n<div>\n<p id=\"subscription-price\">$99.00<\/p>\n<p>only $8.25 per issue<\/p>\n<\/div>\n<p>All prices are NET prices.<br \/> VAT will be added later in the checkout.<br \/>Tax calculation will be finalised during checkout.<\/p>\n<\/div>\n<div>\n<p>Buy article<\/p>\n<p>Get time limited or full article access on ReadCube.<\/p>\n<p id=\"readcube-price\">$32.00<\/p>\n<p>All prices are NET prices.<\/p>\n<\/div>\n<\/section>\n<nav>\n<h3>Additional access options:<\/h3>\n<ul>\n<li>\n            <a href=\"https:\/\/idp.nature.com\/authorize\/natureuser?client_id=grover&#038;redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41587-023-01664-4\" data-track=\"click\" data-track-action=\"login\" data-track-category=\"article body\" data-track-label=\"link\">Log in<\/a>\n        <\/li>\n<li>\n            <a href=\"https:\/\/www.springernature.com\/gp\/librarians\/licensing\/license-options\" data-track=\"click\" data-track-action=\"learn-subscription\" data-track-category=\"article body\" data-track-label=\"link\">Learn about institutional subscriptions<\/a>\n        <\/li>\n<\/ul>\n<\/nav>\n<div data-test=\"figure\" data-container-section=\"figure\" id=\"figure-1\">\n<figure><figcaption><b id=\"Fig1\" data-test=\"figure-caption-text\">Fig. 1: Development of CLASH system for massively parallel knock-in engineering and selection in primary human T cells.<\/b><\/figcaption><div><picture><source type=\"image\/webp\" ><img decoding=\"async\" src=\"http:\/\/media.springernature.com\/m312\/springer-static\/image\/art%3A10.1038%2Fs41587-023-01664-4\/MediaObjects\/41587_2023_1664_Fig1_HTML.png\" alt loading=\"lazy\" width=\"312\" height=\"225\"><\/picture><\/div>\n<\/figure>\n<\/div>\n<div data-enable-entitlement-checks>\n<div id=\"MagazineFulltextArticleBodySuffix\" aria-labelledby=\"Bib1\" data-title=\"References\">\n<h2 id=\"Bib1\">References<\/h2>\n<div data-container-section=\"references\" id=\"Bib1-content\">\n<ol data-track-component=\"outbound reference\">\n<li data-counter=\"1.\">\n<p id=\"ref-CR1\">Dong, M. B. et al. Systematic immunotherapy target discovery using genome-scale in vivo CRISPR screens in CD8 T sells. <i>Cell<\/i> <b>178<\/b>, 1189\u20131204.e1123 (2019).<\/p>\n<p><a data-track=\"click\" rel=\"nofollow noopener\" data-track-label=\"10.1016\/j.cell.2019.07.044\" data-track-action=\"article reference\" href=\"https:\/\/doi.org\/10.1016%2Fj.cell.2019.07.044\" aria-label=\"Article reference 1\" data-doi=\"10.1016\/j.cell.2019.07.044\">Article<\/a>\u00a0<br \/>\n    <a data-track=\"click\" rel=\"nofollow noopener\" data-track-label=\"link\" data-track-action=\"cas reference\" href=\"http:\/\/www.nature.com\/articles\/cas-redirect\/1:CAS:528:DC%2BC1MXhs1Gjur3M\" aria-label=\"CAS reference 1\">CAS<\/a>\u00a0<br \/>\n    <a data-track=\"click\" data-track-action=\"google scholar reference\" data-track-label=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 1\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&#038;title=Systematic%20immunotherapy%20target%20discovery%20using%20genome-scale%20in%20vivo%20CRISPR%20screens%20in%20CD8%20T%20sells&#038;journal=Cell&#038;doi=10.1016%2Fj.cell.2019.07.044&#038;volume=178&#038;pages=1189-1204.e1123&#038;publication_year=2019&#038;author=Dong%2CMB\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<\/li>\n<li data-counter=\"2.\">\n<p id=\"ref-CR2\">Long, L. et al. CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity. <i>Nature<\/i> <b>600<\/b>, 308\u2013313 (2021).<\/p>\n<p><a data-track=\"click\" rel=\"nofollow noopener\" data-track-label=\"10.1038\/s41586-021-04109-7\" data-track-action=\"article reference\" href=\"https:\/\/doi.org\/10.1038%2Fs41586-021-04109-7\" aria-label=\"Article reference 2\" data-doi=\"10.1038\/s41586-021-04109-7\">Article<\/a>\u00a0<br \/>\n    <a data-track=\"click\" rel=\"nofollow noopener\" data-track-label=\"link\" data-track-action=\"cas reference\" href=\"http:\/\/www.nature.com\/articles\/cas-redirect\/1:CAS:528:DC%2BB3MXisFCmt7jE\" aria-label=\"CAS reference 2\">CAS<\/a>\u00a0<br \/>\n    <a data-track=\"click\" data-track-action=\"google scholar reference\" data-track-label=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 2\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&#038;title=CRISPR%20screens%20unveil%20signal%20hubs%20for%20nutrient%20licensing%20of%20T%20cell%20immunity&#038;journal=Nature&#038;doi=10.1038%2Fs41586-021-04109-7&#038;volume=600&#038;pages=308-313&#038;publication_year=2021&#038;author=Long%2CL\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<\/li>\n<li data-counter=\"3.\">\n<p id=\"ref-CR3\">Chen, Z. et al. In vivo CD8<sup>+<\/sup> T cell CRISPR screening reveals control by Fli1 in infection and cancer. <i>Cell<\/i> <b>184<\/b>, 1262\u20131280.e1222 (2021). Refs. 1\u20133 are representative lentivirus-mediated CRISPR screens in primary T cells.<\/p>\n<p><a data-track=\"click\" rel=\"nofollow noopener\" data-track-label=\"10.1016\/j.cell.2021.02.019\" data-track-action=\"article reference\" href=\"https:\/\/doi.org\/10.1016%2Fj.cell.2021.02.019\" aria-label=\"Article reference 3\" data-doi=\"10.1016\/j.cell.2021.02.019\">Article<\/a>\u00a0<br \/>\n    <a data-track=\"click\" rel=\"nofollow noopener\" data-track-label=\"link\" data-track-action=\"cas reference\" href=\"http:\/\/www.nature.com\/articles\/cas-redirect\/1:CAS:528:DC%2BB3MXlsFSgurk%3D\" aria-label=\"CAS reference 3\">CAS<\/a>\u00a0<br \/>\n    <a data-track=\"click\" data-track-action=\"google scholar reference\" data-track-label=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 3\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&#038;title=In%20vivo%20CD8%2B%20T%20cell%20CRISPR%20screening%20reveals%20control%20by%20Fli1%20in%20infection%20and%20cancer&#038;journal=Cell&#038;doi=10.1016%2Fj.cell.2021.02.019&#038;volume=184&#038;pages=1262-1280.e1222&#038;publication_year=2021&#038;author=Chen%2CZ\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<\/li>\n<li data-counter=\"4.\">\n<p id=\"ref-CR4\">Roth, T. L. et al. Pooled knockin targeting for genome engineering of cellular immunotherapies. <i>Cell<\/i> <b>181<\/b>, 728\u2013744.e21 (2020). <b>Mini-pool knock-in in human primary T cells.<\/b><\/p>\n<p><a data-track=\"click\" rel=\"nofollow noopener\" data-track-label=\"10.1016\/j.cell.2020.03.039\" data-track-action=\"article reference\" href=\"https:\/\/doi.org\/10.1016%2Fj.cell.2020.03.039\" aria-label=\"Article reference 4\" data-doi=\"10.1016\/j.cell.2020.03.039\">Article<\/a>\u00a0<br \/>\n    <a data-track=\"click\" rel=\"nofollow noopener\" data-track-label=\"link\" data-track-action=\"cas reference\" href=\"http:\/\/www.nature.com\/articles\/cas-redirect\/1:CAS:528:DC%2BB3cXnsV2it78%3D\" aria-label=\"CAS reference 1\"00>CAS<\/a>\u00a0<br \/>\n    <a data-track=\"click\" data-track-action=\"google scholar reference\" data-track-label=\"link\" rel=\"nofollow noopener\" aria-label=\"CAS reference 1\"11 href=\"http:\/\/scholar.google.com\/scholar_lookup?&#038;title=Pooled%20knockin%20targeting%20for%20genome%20engineering%20of%20cellular%20immunotherapies&#038;journal=Cell&#038;doi=10.1016%2Fj.cell.2020.03.039&#038;volume=181&#038;pages=728-744.e21&#038;publication_year=2020&#038;author=Roth%2CTL\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<\/li>\n<li data-counter=\"5.\">\n<p id=\"ref-CR5\">Schumann, K. et al. Generation of knock-in primary human T cells using Cas9 ribonucleoproteins. <i>Proc. Natl Acad. Sci. USA<\/i> <b>112<\/b>, 10437\u201310442 (2015). <b>CRISPR knock-in in human primary T cells.<\/b><\/p>\n<p><a data-track=\"click\" rel=\"nofollow noopener\" data-track-label=\"10.1073\/pnas.1512503112\" data-track-action=\"article reference\" href=\"https:\/\/doi.org\/10.1073%2Fpnas.1512503112\" aria-label=\"CAS reference 1\"22 data-doi=\"10.1073\/pnas.1512503112\">Article<\/a>\u00a0<br \/>\n    <a data-track=\"click\" rel=\"nofollow noopener\" data-track-label=\"link\" data-track-action=\"cas reference\" href=\"http:\/\/www.nature.com\/articles\/cas-redirect\/1:CAS:528:DC%2BC2MXht1Crt73F\" aria-label=\"CAS reference 1\"33>CAS<\/a>\u00a0<br \/>\n    <a data-track=\"click\" data-track-action=\"google scholar reference\" data-track-label=\"link\" rel=\"nofollow noopener\" aria-label=\"CAS reference 1\"44 href=\"http:\/\/scholar.google.com\/scholar_lookup?&#038;title=Generation%20of%20knock-in%20primary%20human%20T%20cells%20using%20Cas9%20ribonucleoproteins&#038;journal=Proc.%20Natl%20Acad.%20Sci.%20USA&#038;doi=10.1073%2Fpnas.1512503112&#038;volume=112&#038;pages=10437-10442&#038;publication_year=2015&#038;author=Schumann%2CK\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<\/li>\n<\/ol>\n<p><a data-track=\"click\" data-track-action=\"download citation references\" data-track-label=\"link\" rel=\"nofollow\" href=\"https:\/\/citation-needed.springer.com\/v2\/references\/10.1038\/s41587-023-01664-4?format=refman&#038;flavour=references\">Download references<\/a><\/p>\n<\/div>\n<\/div>\n<div id=\"additional-information-section\" data-title=\"Additional information\">\n<h2 id=\"additional-information\">Additional information<\/h2>\n<div id=\"additional-information-content\">\n<p><b>Publisher\u2019s note<\/b> Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.<\/p>\n<p><b>This is a summary of:<\/b> Dai, X. et al. Massively parallel knock-in engineering of human T cells. <i>Nat. 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1.3zm3.01 2.23c.31.24.61.43.92.57.3.13.63.2.98.2.38 0 .65-.08.83-.23s.27-.35.27-.6c0-.14-.05-.26-.13-.37-.08-.1-.2-.2-.34-.28-.14-.09-.29-.16-.47-.23l-.53-.22c-.23-.09-.46-.18-.69-.3-.23-.11-.44-.24-.62-.4s-.33-.35-.45-.55c-.12-.21-.18-.46-.18-.75 0-.61.23-1.1.68-1.49.44-.38 1.06-.57 1.83-.57.48 0 .91.08 1.29.25s.71.36.99.57l-.74.98c-.24-.17-.49-.32-.73-.42-.25-.11-.51-.16-.78-.16-.35 0-.6.07-.76.21-.17.15-.25.33-.25.54 0 .14.04.26.12.36s.18.18.31.26c.14.07.29.14.46.21l.54.19c.23.09.47.18.7.29s.44.24.64.4c.19.16.34.35.46.58.11.23.17.5.17.82 0 .3-.06.58-.17.83-.12.26-.29.48-.51.68-.23.19-.51.34-.84.45-.34.11-.72.17-1.15.17-.48 0-.95-.09-1.41-.27-.46-.19-.86-.41-1.2-.68z" fill="#535353"/></g></svg>\"><\/a><\/p>\n<div>\n<h3 id=\"citeas\">Cite this article<\/h3>\n<p> CLASH enables large-scale parallel knock-in for cell engineering.<br \/>\n                    <i>Nat Biotechnol<\/i>  (2023). https:\/\/doi.org\/10.1038\/s41587-023-01664-4<\/p>\n<p><a data-test=\"citation-link\" data-track=\"click\" data-track-action=\"download article citation\" data-track-label=\"link\" data-track-external rel=\"nofollow\" href=\"https:\/\/citation-needed.springer.com\/v2\/references\/10.1038\/s41587-023-01664-4?format=refman&#038;flavour=citation\">Download citation<\/a><\/p>\n<ul data-test=\"publication-history\">\n<li>\n<p>Published<span>: <\/span><span><time datetime=\"2023-01-26\">26 January 2023<\/time><\/span><\/p>\n<\/li>\n<li>\n<p><abbr title=\"Digital Object Identifier\">DOI<\/abbr><span>: <\/span><span>https:\/\/doi.org\/10.1038\/s41587-023-01664-4<\/span><\/p>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div><\/div>\n<\/p><\/div>\n<p><a href=\"https:\/\/www.nature.com\/articles\/s41587-023-01664-4\" class=\"button purchase\" rel=\"nofollow noopener\" target=\"_blank\">Read More<\/a><br \/>\n Laine Byron<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Research Briefing Published: 26 January 2023 Nature Biotechnology (2023)Cite this article 369 Accesses 1 Altmetric Metrics details Subjects CLASH is a CRISPR-based platform that enables the parallel knock-in via homology-directed repair of a large pool of transgene variants encoded in adeno-associated virus vectors. CLASH can be applied to the systematic and unbiased selection of favorable<\/p>\n","protected":false},"author":1,"featured_media":602604,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1531,37732,536],"tags":[],"class_list":{"0":"post-602603","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-clash","8":"category-enables","9":"category-science-nature"},"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/posts\/602603","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/comments?post=602603"}],"version-history":[{"count":0,"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/posts\/602603\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/media\/602604"}],"wp:attachment":[{"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/media?parent=602603"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/categories?post=602603"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/tags?post=602603"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}