{"id":596175,"date":"2023-01-10T09:59:57","date_gmt":"2023-01-10T15:59:57","guid":{"rendered":"https:\/\/news.sellorbuyhomefast.com\/index.php\/2023\/01\/10\/princeton-chemists-create-quantum-dots-at-room-temperature-using-custom-protein\/"},"modified":"2023-01-10T09:59:57","modified_gmt":"2023-01-10T15:59:57","slug":"princeton-chemists-create-quantum-dots-at-room-temperature-using-custom-protein","status":"publish","type":"post","link":"https:\/\/newsycanuse.com\/index.php\/2023\/01\/10\/princeton-chemists-create-quantum-dots-at-room-temperature-using-custom-protein\/","title":{"rendered":"Princeton Chemists Create Quantum Dots at Room Temperature Using Custom Protein"},"content":{"rendered":"<div>\n<div id=\"attachment_242406\">\n<p><img fetchpriority=\"high\" aria-describedby=\"caption-attachment-242406\" decoding=\"async\" src=\"https:\/\/scitechdaily.com\/images\/Aqueous-Solution-of-Quantum-Dots-Luminescence.jpg?ezimgfmt=ng%3Awebp%2Fngcb2%2Frs%3Adevice%2Frscb2-1\" alt=\"Aqueous Solution of Quantum Dots Luminescence\" width=\"777\" height=\"518\" srcset sizes ezimgfmt=\"rs rscb2 src ng ngcb2 srcset\" loading=\"eager\" importance=\"high\"><\/p>\n<p id=\"caption-attachment-242406\">Professor Michael Hecht and his research group at Princeton have made a significant discovery in the field of chemistry by creating the first known de novo protein that catalyzes the synthesis of quantum dots. Quantum dots are nanocrystals with fluorescent properties that are used in a range of electronic applications, including LED screens and solar panels. This new method of creating quantum dots has the potential to be more sustainable and environmentally friendly than current methods, as it demonstrates that functional materials can be synthesized using protein sequences that are not derived from nature.<\/p>\n<\/div>\n<p><strong>Researchers at Princeton\u2019s Department of Chemistry discovered the first known de novo protein that catalyzes, or drives, the synthesis of quantum dots.<\/strong><\/p>\n<p>Nature uses 20 canonical <span aria-describedby=\"tt\" data-cmtooltip=\"\n\n<div class=glossaryItemTitle>amino acids<\/div>\n<div class=glossaryItemBody>&lt;div class=&quot;cell text-container large-6 small-order-0 large-order-1&quot;&gt;<br \/>\n&lt;div class=&quot;text-wrapper&quot;&gt;&lt;br \/&gt;Amino acids are a set of organic compounds used to build proteins. There are about 500 naturally occurring known amino acids, though only 20 appear in the genetic code. Proteins consist of one or more chains of amino acids called polypeptides. The sequence of the amino acid chain causes the polypeptide to fold into a shape that is biologically active. The amino acid sequences of proteins are encoded in the genes. Nine proteinogenic amino acids are called &quot;essential&quot; for humans because they cannot be produced from other compounds by the human body and so must be taken in as food.&lt;br \/&gt;&lt;\/div&gt;<br \/>\n&lt;\/div&gt;<\/div>\n<p>&#8221; data-gt-translate-attributes=&#8221;[{&#8220;attribute&#8221;:&#8221;data-cmtooltip&#8221;, &#8220;format&#8221;:&#8221;html&#8221;}]&#8221;>amino acids<\/span> as building blocks to make proteins, combining their sequences to create complex molecules that perform biological functions.<\/p>\n<p>But what happens with the sequences <em>not<\/em> selected by nature? And what possibilities lie in constructing entirely new sequences to make novel, or <em>de novo,<\/em> proteins bearing little resemblance to anything in nature?<\/p>\n<div id=\"attachment_242382\">\n<p><img aria-describedby=\"caption-attachment-242382\" decoding=\"async\" loading=\"lazy\" alt=\"Quantum Dots Electron Microscope\" width=\"777\" height=\"777\" srcset=\"https:\/\/scitechdaily.com\/images\/Quantum-Dots-Electron-Microscope-777x777.jpg 777w,https:\/\/scitechdaily.com\/images\/Quantum-Dots-Electron-Microscope-400x400.jpg 400w,https:\/\/scitechdaily.com\/images\/Quantum-Dots-Electron-Microscope-150x150.jpg 150w,https:\/\/scitechdaily.com\/images\/Quantum-Dots-Electron-Microscope-768x768.jpg 768w,https:\/\/scitechdaily.com\/images\/Quantum-Dots-Electron-Microscope-120x120.jpg 120w,https:\/\/scitechdaily.com\/images\/Quantum-Dots-Electron-Microscope.jpg 1400w\"  ezimgfmt=\"rs rscb2 src ng ngcb2 srcset\" src=\"https:\/\/scitechdaily.com\/images\/Quantum-Dots-Electron-Microscope-777x777.jpg\" ><\/p>\n<p id=\"caption-attachment-242382\">An image of quantum dots taken under an electron microscope. These quantum dots were produced in the Hecht Lab using de novo proteins. Each of them is 2 nanometers in diameter, an important factor since particle size determines the color they glow, or fluoresce, in. Credit: Courtesy of the Hecht and Scholes labs<\/p>\n<\/div>\n<p>That\u2019s the terrain where Michael Hecht, professor of chemistry, works with his research group. And recently, their curiosity for designing their own sequences paid off.<\/p>\n<p>They discovered the first known <em>de novo<\/em> (newly created) protein that catalyzes, or drives, the synthesis of quantum dots. Quantum dots are fluorescent nanocrystals used in electronic applications from LED screens to solar panels.<\/p>\n<p>Their work opens the door to making nanomaterials in a more sustainable way by demonstrating that protein sequences not derived from nature can be used to synthesize functional materials \u2014 with pronounced benefits to the environment.<\/p>\n<p><iframe loading=\"lazy\" title=\"Princeton Innovation 2022: Sustainable quantum dot production, Michael Hecht\" width=\"777\" height=\"437\" data-ezsrc=\"https:\/\/www.youtube.com\/embed\/R_tmuXA6fdE?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" allowfullscreen><\/iframe><\/p>\n<p>Quantum dots are normally made in industrial settings with high temperatures and toxic, expensive solvents \u2014 a process that is neither economical nor environmentally friendly. But Hecht and his research group pulled off the process in the lab using water as a solvent, making a stable end-product at room temperature.<\/p>\n<p>\u201cWe\u2019re interested in making life molecules, proteins, that did not arise in life,\u201d said Hecht, who led the research with Greg Scholes, the William S. Tod Professor of Chemistry and chair of the department. \u201cIn some ways we\u2019re asking, are there alternatives to life as we know it? All life on earth arose from common ancestry. But if we make lifelike molecules that did not arise from common ancestry, can they do cool stuff?<\/p>\n<p>\u201cSo here, we\u2019re making novel proteins that never arose in life doing things that don\u2019t exist in life.\u201d<\/p>\n<p>The team\u2019s process can also tune nanoparticle size, which determines the color quantum dots glow, or fluoresce, in. That holds possibilities for tagging molecules within a biological system, like staining cancer cells <em>in vivo.<\/em><\/p>\n<div id=\"attachment_242381\">\n<p><img aria-describedby=\"caption-attachment-242381\" decoding=\"async\" loading=\"lazy\" alt=\"Michael Hecht and Yueyu Yao\" width=\"777\" height=\"622\" srcset=\"https:\/\/scitechdaily.com\/images\/Michael-Hecht-and-Yueyu-Yao-777x622.jpg 777w,https:\/\/scitechdaily.com\/images\/Michael-Hecht-and-Yueyu-Yao-400x320.jpg 400w,https:\/\/scitechdaily.com\/images\/Michael-Hecht-and-Yueyu-Yao-768x614.jpg 768w,https:\/\/scitechdaily.com\/images\/Michael-Hecht-and-Yueyu-Yao-1536x1229.jpg 1536w,https:\/\/scitechdaily.com\/images\/Michael-Hecht-and-Yueyu-Yao-2048x1638.jpg 2048w\"  ezimgfmt=\"rs rscb2 src ng ngcb2 srcset\" src=\"https:\/\/scitechdaily.com\/images\/Michael-Hecht-and-Yueyu-Yao-777x622.jpg\" ><\/p>\n<p id=\"caption-attachment-242381\">Professor Michael Hecht, and fifth-year graduate student and co-author on the quantum dot research Yueyu Yao, in Frick Laboratory. Credit: Photo by Jesse Condon<\/p>\n<\/div>\n<p>\u201cQuantum dots have very interesting optical properties due to their sizes,\u201d said Yueyu Yao, co-author on the paper and a fifth-year graduate student in Hecht\u2019s lab. \u201cThey\u2019re very good at absorbing light and converting it to chemical energy \u2014 that makes them useful for being made into solar panels or any sort of photo sensor.<\/p>\n<p>\u201cBut on the other hand, they\u2019re also very good at emitting light at a certain desired wavelength, which makes them suitable for making LED screens.\u201d<\/p>\n<p>And because they\u2019re small \u2014 composed of only about 100 atoms and maybe 2 nanometers across \u2014 they\u2019re able to penetrate some biological barriers, making their utility in medicines and biological imaging especially promising.<\/p>\n<h4>Why use <em>de novo<\/em> proteins?<\/h4>\n<p>\u201cI think using <em>de novo<\/em> proteins opens up a way for designability,\u201d said Leah Spangler, lead author on the research and a former postdoc in the Scholes Lab. \u201cA key word for me is \u2018engineering.\u2019 I want to be able to engineer proteins to do something specific, and this is a type of protein you can do that with.<\/p>\n<p>\u201cThe quantum dots we\u2019re making aren\u2019t great quality yet, but that can be improved by tuning the synthesis,\u201d she added. \u201cWe can achieve better quality by engineering the protein to influence quantum dot formation in different ways.\u201d<\/p>\n<div id=\"attachment_242385\">\n<p><img aria-describedby=\"caption-attachment-242385\" decoding=\"async\" loading=\"lazy\" alt=\"Leah Spangler\" width=\"777\" height=\"541\" srcset=\"https:\/\/scitechdaily.com\/images\/Leah-Spangler-777x541.jpg 777w,https:\/\/scitechdaily.com\/images\/Leah-Spangler-400x278.jpg 400w,https:\/\/scitechdaily.com\/images\/Leah-Spangler-768x534.jpg 768w,https:\/\/scitechdaily.com\/images\/Leah-Spangler.jpg 960w\"  ezimgfmt=\"rs rscb2 src ng ngcb2 srcset\" src=\"https:\/\/scitechdaily.com\/images\/Leah-Spangler-777x541.jpg\" ><\/p>\n<p id=\"caption-attachment-242385\">Leah Spangler, lead author on the paper, in Frick Lab last year. Credit: Photo by<br \/>C. Todd Reichart, Department of Chemistry<\/p>\n<\/div>\n<p>Based on work done by corresponding author Sarangan Chari, a senior chemist in Hecht\u2019s lab, the team used a <em>de novo<\/em> protein it designed named ConK to catalyze the reaction. Researchers first isolated ConK in 2016 from a large combinatorial library of proteins. It\u2019s still made of natural amino acids, but it qualifies as \u201c<em>de novo<\/em>\u201d because its sequence doesn\u2019t have any similarity to a natural protein.<\/p>\n<p>Researchers found that ConK enabled the survival of <em>E. coli<\/em> in otherwise toxic concentrations of copper, suggesting it might be useful for metal binding and sequestration. The quantum dots used in this research are made out of cadmium sulfide. Cadmium is a metal, so researchers wondered if ConK could be used to synthesize quantum dots.<\/p>\n<p>Their hunch paid off. ConK breaks down cysteine, one of the 20 amino acids, into several products, including hydrogen sulfide. That acts as the active sulfur source that will then go on to react with the metal cadmium. The result is CdS quantum dots.<\/p>\n<p>\u201cTo make a cadmium sulfide quantum dot, you need the cadmium source and the sulfur source to react in solution,\u201d said Spangler. \u201cWhat the protein does is make the sulfur source slowly over time. So, we add the cadmium initially but the protein generates the sulfur, which then reacts to make distinct sizes of quantum dots.\u201d<\/p>\n<p>Reference: \u201cA de novo protein catalyzes the synthesis of semiconductor quantum dots\u201d by Leah C. Spangler, Yueyu Yao, Guangming Cheng, Nan Yao, Sarangan L. Chari, Gregory D. Scholes and Michael H. Hecht, 12 December 2022, <em>Proceedings of the National Academy of Sciences<\/em>.<br \/><a href=\"https:\/\/doi.org\/10.1073\/pnas.2204050119\">DOI: 10.1073\/pnas.2204050119<\/a><\/p>\n<p><em>This research was supported by the National Science Foundation MRSEC program (DMR-2011750), the <span aria-describedby=\"tt\" data-cmtooltip=\"\n\n<div class=glossaryItemTitle>Princeton University<\/div>\n<div class=glossaryItemBody>Founded in 1746, Princeton University is a private Ivy League research university in Princeton, New Jersey and the fourth-oldest institution of higher education in the United States. It provides undergraduate and graduate instruction in the humanities, social sciences, natural sciences, and engineering.<\/div>\n<p>&#8221; data-gt-translate-attributes=&#8221;[{&#8220;attribute&#8221;:&#8221;data-cmtooltip&#8221;, &#8220;format&#8221;:&#8221;html&#8221;}]&#8221;>Princeton University<\/span> Writing Center and the Canadian Institute for Advanced Research. The research was also supported by NSF grant MCB-1947720 to MH.<\/em><\/p>\n<\/div>\n<p><a href=\"https:\/\/scitechdaily.com\/princeton-chemists-create-quantum-dots-at-room-temperature-using-custom-protein\/\" class=\"button purchase\" rel=\"nofollow noopener\" target=\"_blank\">Read More<\/a><br \/>\n Princeton University<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Professor Michael Hecht and his research group at Princeton have made a significant discovery in the field of chemistry by creating the first known de novo protein that catalyzes the synthesis of quantum dots. Quantum dots are nanocrystals with fluorescent properties that are used in a range of electronic applications, including LED screens and solar<\/p>\n","protected":false},"author":1,"featured_media":596176,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[22168,31523,536],"tags":[],"class_list":["post-596175","post","type-post","status-publish","format-standard","has-post-thumbnail","category-chemists","category-princeton","category-science-nature"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/posts\/596175","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/comments?post=596175"}],"version-history":[{"count":0,"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/posts\/596175\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/media\/596176"}],"wp:attachment":[{"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/media?parent=596175"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/categories?post=596175"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/newsycanuse.com\/index.php\/wp-json\/wp\/v2\/tags?post=596175"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}