Base editors

US11319532B2

Strategies, systems, reagents, methods and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject; for example, within the human genome. In one embodiment, fusion proteins comprise a Gam protein, a nucleic acid programmable DNA binding protein and a cytidine deaminase.

President and Fellows of Harvard College (Cambridge, MA, USA)

Liu DR, Zhao KT, Kim Y

5/3/2022

AU2016342380B2

Strategies, systems, reagents, methods and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject; for example, within the human genome. In one embodiment, fusion proteins of Cas9 and nucleic acid editing proteins or protein domains — for example, deaminase domains — are provided.

President and Fellows of Harvard College (Cambridge, MA, USA)

Kim Y, Komor AC, Liu DR, Rees HA

4/7/2022

US11268082B2

Strategies, systems, reagents, methods and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject; for example, within the human genome. In one embodiment, fusion proteins of nucleic acid programmable DNA binding proteins — for example, Cpf1 or variants thereof — and nucleic acid editing proteins or protein domains — for example, deaminase domains — are provided.

President and Fellows of Harvard College (Cambridge, MA, USA)

Liu DR, Komor AC, Chen L, Rees HA

3/8/2022

CN109021111B

A fusion expressed protein (base editor) of human apolipoprotein B messenger RNA deaminase catalytic subunit 3A (APOBEC3A), CRISPR-related Cas protein and uracil glycosidase inhibitor (UGI). The base editor can edit bases in DNA, deaminates cytosine into uracil, and has high editing efficiency even if cytosine is positioned at a GpC site or is in a hypermethylated state.

ShanghaiTech University (Shanghai, China)

Chen J, Yang L, Huang X, Yang B, Wang X, Li J

12/7/2021

US10947530B2

Adenosine deaminases that are capable of deaminating adenosine in DNA. Also, fusion proteins comprising a Cas9 (for example, a Cas9 nickase) domain and adenosine deaminases that deaminate adenosine in DNA. In some embodiments, the fusion proteins further comprise a nuclear localization sequence and/or an inhibitor of base repair, such as, a nuclease-dead inosine-specific nuclease.

President and Fellows of Harvard College (Cambridge, MA, USA)

Liu DR, Gaudelli N

3/16/2021

CN106916852B

A base-editing system and a construction and application method thereof. The base-editing system is characterized by comprising a UGI expression vector and a BE3 expression vector or a UGI and BE3 co-expression vector. The invention increases the C–T editing rate generated by the CRISPR base editor and reduces the base insertion or deletion rate caused by the CRISPR system, thereby enhancing the efficacy of the CRISPR base editor and providing a new method for implementing more accurate and safe base editing in genomes of various species by the CRISPR base editor.

ShanghaiTech University (Shanghai, China)

Chen J, Yang L, Yang B, Xue W, Wang L

12/4/2020

KR102084186B1

A composition for inducing DNA single-strand breaks, comprising a cytidine deaminase, an inactivated target-specific endonuclease, and a guide RNA; a method for inducing a single-strand break in DNA, using the same; a method for analyzing the nucleic acid sequence of a base-editing-introduced DNA; and a method for identifying (or measuring or detecting) a base-editing site or base-editing efficiency at an on-target site, an off-target site and/or target specificity.

Institute for Basic Science (Daejon, S. Korea)

Kim JS

3/3/2020