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CLASH is a CRISPR-based platform that enables the parallel knock-in via homology-directed repair of a large pool of transgene variants encoded in adeno-associated virus vectors. CLASH can be applied to the systematic and unbiased selection of favorable features in T cells and, in principle, other cell types.
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References
Dong, M. B. et al. Systematic immunotherapy target discovery using genome-scale in vivo CRISPR screens in CD8 T sells. Cell 178, 1189–1204.e1123 (2019).
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Chen, Z. et al. In vivo CD8+ T cell CRISPR screening reveals control by Fli1 in infection and cancer. Cell 184, 1262–1280.e1222 (2021). Refs. 1–3 are representative lentivirus-mediated CRISPR screens in primary T cells.
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This is a summary of: Dai, X. et al. Massively parallel knock-in engineering of human T cells. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01639-x (2023).
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CLASH enables large-scale parallel knock-in for cell engineering.
Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01664-4
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DOI: https://doi.org/10.1038/s41587-023-01664-4
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