- News & Views
- Published:
Single-cell technology
(2023)Cite this article
180 Accesses
18 Altmetric
Subjects
Elongating protein fibers record the transcriptional activity of single cells for later readout by imaging.
Previous studies have demonstrated the feasibility of encoding cellular history in the form of nucleic acid. However, analysis of DNA or RNA sequence modifications requires cell lysis, which eliminates spatial information. In contrast, the protein-based methods of Lin et al. and Linghu et al. retain spatial information, enabling activity mapping in complex cellular populations in the context of intact tissues.
This is a preview of subscription content, access via your institution
Access options
Subscribe to Nature+
Get immediate online access to Nature and 55 other Nature journal
Subscribe to Journal
Get full journal access for 1 year
$99.00
only $8.25 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Tax calculation will be finalised during checkout.
Buy article
Get time limited or full article access on ReadCube.
$32.00
All prices are NET prices.
References
Chen, T. W. et al. Nature 499, 295–300 (2013).
Park, Y. G. et al. Nat. Biotechnol. https://doi.org/10.1038/nbt.4281 (2018).
Renier, N. et al. Cell 165, 1789–1802 (2016).
Lin, D. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01524-7 (2022).
Linghu, C. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01586-7 (2022).
Baskaran, Y. et al. Nat. Commun. 6, 8681 (2015).
Garcia-Seisdedos, H., Empereur-Mot, C., Elad, N. & Levy, E. D. Nature 548, 244–247 (2017).
Lee, D., Hyun, J. H., Jung, K., Hannan, P. & Kwon, H. B. Nat. Biotechnol. 35, 858–863 (2017).
Wang, W. et al. Nat. Biotechnol. 35, 864–871 (2017).
Ethics declarations
Competing interests
The author declares no competing interests.
Rights and permissions
About this article
Cite this article
Kwon, HB. Writing cellular history in protein chains.
Nat Biotechnol (2023). https://doi.org/10.1038/s41587-022-01597-4
Published:
DOI: https://doi.org/10.1038/s41587-022-01597-4
Associated Content
Read More
Hyung-Bae Kwon